Hauv thaj teb ntawm BiomileS kev tshawb fawb, hydrogels, vim lawv cov khoom muag muag thiab dej cov ntsiab lus, tau ua cov ntaub ntawv tseem ceeb ntawm cov khoom siv ntse thiab tshuaj kho mob. N {- vinylprolidone (NVP) COPLORSITY, nrog rau lawv cov cim hydrophilitive hauv cov ntawv lo ntsiab muag thiab kev tshuaj ntsuam xyuas. Tom ntej, Kuv yuav xyuas cov qauv {{}} cov cuab yeej sib raug zoo ntawm cov tshuab hluav taws xob, pib los ntawm lawv cov synthesis mechanism. Kuv tseem yuav delve mus rau hauv lawv cov ntawv thov yuav muaj nyob rau hauv biomedical nqis, muab ib qho siv tau zoo rau kev txhim kho ntawm ntau lub multifunctional biomaterials.
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1. Precision tswj cov khoom siv hluavtaws thiab cov khoom siv
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2. Cov qauv tsom xam ntawm cov qauv -}} kev sib txuam
2.1 molecular txheej txheem ntawm kev o thiab kev teb ib puag ncig
2.2 cov ntaub ntawv uas twb muaj lawm ntawm cov dej lwg me thiab cov khoom thauj
2.3 Interfacial tus cwj pwm thiab tswj cov tswv yim ntawm cov protein adsorption -
3. Kev nthuav dav thiab cov nyom ntawm cov ntawv thov biomedical
1. Precision tswj cov khoom siv hluavtaws thiab cov khoom siv
Cov synthesis ntawmNvp copolymerHydrogels cuam tshuam nrog cov txheej txheem synergistic ntawm dawb radical polymerization thiab kev siv network. Hloov maj mam kho kom haum rau cov kev tshwm sim ncaj qha cuam tshuam cov khoom siv. Cov kev sim tau qhia tau tias thaum siv AzobisisobutYronitrile (ABNN) raws li kev pib ua kom tau nruj rau 0.05% {12} {0.1% ntawm tag nrho cov monomer. Kev tshuaj ntau dhau ntawm cov txiaj ntsig tsis tiav polymerization, nrog hloov dua siab tshiab hauv qab 50%. Qhov kev siv tshuaj ntau dhau siab dawb radical kev sib nraus, ua rau ib cheeb tsam dawb thiab tsis sib xws crosslinking hauv cov khoom. Ua kom zoo rau cov tshuaj tiv thaiv kub kuj tseem ceeb heev. Ib qho kub ntawm 50-70 kev sib npaug sib npaug kev ua haujlwm thiab monomer kev ua ub no. Nyob rau ntawm 70 degree, lub coppymerization ntawm nvp nrog -hydroxyethyl methacrylate (Hema) ua tiav qhov kev hloov pauv siab tshaj plaws, mus txog 93.2%, thiab cov qauv kev sib txuas lus ntau tshaj plaws.
Los ntawm kev kho NVP rau Hema Piv (0: 100 txog 40:60), Gradient tswj cov khoom muaj peev xwm ua tiav. Thaum cov NVP cov ntsiab lus tso nyiaj rau 20% los ntawm qhov hnyav, cov hydrogel ua tau zoo tshaj plaws ntawm 96.3%, sib ntsib cov kev paub txog kev tawm ntawm cov ntsiab muag. Nws cov ntawv sau ua kom ruaj khov tseem nyob ruaj khov ntawm 1.3364, txuas ze rau tib neeg lub Carta lub chaw ua haujlwm tsis zoo (1.3375), txo qhov views views. Tus neeg sib cuag ib qho txo qis los ntawm 40℃rau 32 degree, txhim kho hydrophilicity hydrophilicity thiab ua kom muaj kev sib txawv ntawm lub lens thiab lub qhov muag.
Cov ntaub ntawv 'Biosafety yog tus taw qhia tseem ceeb rau kev siv tshuaj kho mob. Cov tshuaj cyotoxicity qhia tau tias cov hydrogel extract ua kom pom tseeb tus nqi (rgrcs) tshaj li 90%, ua tiav qhov kev ntsuas toxicity ntawm qib 1, uas tsis muaj cov nyhuv cytostatic. Tus rho tawm cov hom yuav ruaj khov nyob ib ncig ntawm 7.2, yuav luag zoo ib yam rau tib neeg lub kua muag (PH 7.5), tiv thaiv {}} Basem tsis txaus ntseeg nrog lub sijhawm ntev hnav. Cov tshuaj tiv thaiv cov kev ntsuam xyuas paub meej tias cov khoom tseem nyob ruaj khov nyob rau hauv cov qauv keeb kwm xws li ethanol thiab cov acetone rau 30 hnub, yooj yim rau kev siv tshuaj tua kab mob txuas ntxiv thiab cia.
2. Cov qauv tsom xam ntawm cov qauv -}} kev sib txuam
2.1 molecular txheej txheem ntawm kev o thiab kev teb ib puag ncig
O o o oferties ntawmNvp copolymerHydrogels yog ib tus cwj pwm tseem ceeb ntawm lawv cov kev yoog raws li cov khoom siv hluav taws xob thiab tau txiav txim siab los ntawm ob qho tib si lub network hydrophilicity thiab crosslink ntom. Qhov kev sib npaug ntawm cov ncauj lus dej sib npaug (EWC) nce linearly nrog NVP cov ntsiab lus. Raws li NVP cov ntsiab lus nce los ntawm 0 txog 40%, EWC sawv ntawm 37.40% rau 76.40%. Qhov no yog ntaus nqi rau cov pab pawg uas lom zem ({{}}}}}}}}}}}}}}}}} Dynamic o kev sim ua kom pom tias cov khoom mus txog o equilibrium hauv dej distilled tom qab 24 teev. Kev qhia txog ntawm hydrophobic monomer n}} butyl mchacrylate (bma) txo qhov kev tswj hwm kom tiv thaiv tau thiab muaj txiaj ntsig zoo rau kev tswj hwm lub sijhawm ntawm cov khoom qab zib resture.
Cov khoom siv tau luag haujlwm ntawm cov txheej txheem sab nraud los ntawm cov pab pawg ntawm hydrophobic (ua rau lub network kom txo qis hauv o dua 35 qib; Thaum tus nqi p tus nqi rau 4.13, cov tswv cuab ntawm cov pab pawg carboxyl nce ntxiv ntawm cov ntu ntu, thiab cov qib o nce li 25% piv rau nruab nrab ib puag ncig; Kev cuam tshuam ntawm ionic lub zog yog ua tiav los ntawm "salting tawm cov nyhuv", thiab 0.3 Mol / l NacL daws yuav txo tau qhov o kom 40%. Cov cuab yeej no tuaj yeem sib phim cov kev hloov pauv ntawm ib puag ncig ionic ntawm tib neeg lub cev.
2.2 cov ntaub ntawv uas twb muaj lawm ntawm cov dej lwg me thiab cov khoom thauj
Lub xeev ntawm cov dej hauv cov gel network ncaj qha cuam tshuam rau kev siv tshuab thiab permeability cov khoom ntawm cov khoom. Cov tshuaj sib txawv uas yog muaj peb hom dej hauv hydrogenary (tsis muaj zog ntawm cov dej khov kho (tsis muaj zog) Dej). Nonfreezing ciam dej nyiaj rau 9.22% mus rau 16.01% thiab ua raws li lub tshuab yas ntim hauv lub network. Ua kom nws cov ncauj lus txo qis qis dua lub tensile lub zog ntawm cov khoom siv los ntawm 925 kpa rau 406 kPa, tab sis nce nws zoo ib yam li cov khoom siv mechanical ntawm pob txha nqaij nqaij.
Cov pa oxygen thiab ion thauj khoom yog cov ntsuas kev ua tau zoo rau cov ntsiab muag hu. Kev tshawb fawb tau lees tias cov dej dawb yog thawj qhov nruab nrab rau huab hwm coj hloov. Rau txhua 10% ntxiv hauv dej dawb, cov oxygen permeability coefficient (DK) nce ntxiv ntawm 8 × 10⁻⁴ ml / cm² · h. Qhov txawv coefficients ntawm poov tshuaj thiab sodium ions yog linearly cuam tshuam nrog cov kev xav "ntim dawb." Thaum h=0.6, cov kev sib txawv coefficients ntawm K⁺ thiab Na⁺ ncav cuag 5.50 × 10⁻⁶ ×⁻⁶⁻⁶tte sib npaug hauv lub qhov muag.
2.3 Interfacial tus cwj pwm thiab tswj cov tswv yim ntawm cov protein adsorption
Qhov kev tso tawm ntawm cov kua muag ntawm cov chaw hydrogel yog qhov teeb meem tseem ceeb cuam tshuam rau lub neej ntawm cov iav ci. Cov kev tshawb fawb siv Bovine Serum albumin (BSA) raws li cov qauv adsorption ntau ntxiv rau cov ntsiab lus ntawm Langmuor, thiab nce mus 110 mg / g ntawm 30% NVP cov ntsiab lus. Qhov no yog vim muaj ntau yam kev sib cuam tshuam ntawm cov pab pawg hauv cov pab pawg hauv NVP lwg me me thiab cov cheeb tsam hydrothobic ntawm cov protein ntawm cov protein. Tsis tas li ntawd, ionic hydrogelels nthuav tawm 40% ntau dua kev muaj peev xwm muaj peev xwm muaj peev xwm tsis muaj hydrogs vim yog tus nqi nyiam.
Qhov cuam tshuam ntawm ib puag ncig ntawm adsorption kev qhia cov qauv niaj zaus: thaum lub sijhawm kub thiab txias, muaj peev xwm ua kom muaj peev xwm ua tiav los ntawm 15%. Thaum lub pH mus txog ntawm isoelectric taw tes ntawm BSA (4.7), intrololecular repulsion yog tsawg, thiab adsorption muaj peev xwm nce nws lub ncov. Ua kom muaj lub zog ionic (0.1-0.3 mol / l nacl) txhawb kev cuam tshuam hydrophobic cuam tshuam los ntawm kev tiv thaiv kev tiv thaiv los ntawm 25%. Nws yog tsim nyog sau tseg tias cov adsorbed protein yuav thaiv cov channel network, ua rau muaj cov pa roj oxygen, txo qis hauv cov lej "txo qis hauv IAf diffusion tus nqi. Yog li ntawd, nws yog ib qho tsim nyog yuav txo cov adspecific los ntawm kev hloov kho saum npoo (xws li qhia polyethylene glycol spegments).
3. Kev nthuav dav thiab cov nyom ntawm cov ntawv thov biomedical
Hauv kev sib chwv lub laub ua lag luam, cov hydrogel no muaj cov txiaj ntsig tseem ceeb hauv kev ua haujlwm kho qhov muag thiab xis nyob. Piv rau cov tshuaj polmethyl me me (PMMA) cov ntsiab muag, nws cov pa oxygen, nws lub sijhawm tiv thaiv tau zoo los ntawm hypoxia. Nws cov nyhuv moisturizing yog txuas ntxiv rau ntau teev, txo kev tsis xis nyob rau cov neeg mob nrog lub qhov muag qhuav. Kev sim ua ntej tau qhia tias tom qab 30 hnub ntawm Lens Nruam Nrov tau pom, thiab Interletinatory teb tau pom, thiab interleUk-6) kev txhawj xeeb nyob rau hauv cov kua txwv tseem nyob ntawm cov qib li qub (<10 pg/mL).
Hauv kev tswj hwm cov tshuaj kho yeeb yaj kiab, cov khoom siv ib puag ncig ua kom muaj kev txawj siv tshuaj yeeb. Piv txwv li, thaum anti -} qhov fluorometholone yog thauj mus rau hauv hydrogel, thaum ocular mob ua rau ib tee hauv ph (<7.0), the network swelling increases, and the drug release rate doubles. Once the inflammation subsides and the pH rises again, the release rate automatically decreases, enabling "on-demand drug delivery." Furthermore, its porous network structure can load growth factors, slowly releasing them during wound repair, promoting corneal epithelial cell proliferation and achieving a healing rate 1.5 times faster than traditional dressings.
Cov kev tshawb nrhiav tam sim no tseem muaj cov kev sib tw dej ntau nrog cov khoom siv tshuab hluav taws xob muaj zog (cov khoom siv dej> 70% ua rau cov protein ntxiv kom ntev dua lens lub neej. Kev tshawb fawb yav tom ntej tuaj yeem qhia cov ntu nruj los ntawm kev tswj hwm network (IPN) los ua kom pom tseeb cov khoom siv rau cov khoom siv tshuaj ntsuam xyuas.
Kev tshawb fawb txog NVP copolymer hydrogels qhia cov kev sib cuam tshuam zoo ntawm cov kev sib txuas lus polymer thiab kev sib txuas lus lom neeg. Nws hloov ntawm kev sim tshuaj schthesis rau cov ntawv thov kho mob embodies sib sib zog nqus ntawm cov ntaub ntawv kev tshawb fawb thiab kev xav tau kev kho mob. Nrog kev sib tawg hauv cov txheej txheem synthesis thiab kev hloov kho ua haujlwm zoo, cov ntaub ntawv kho tshiab rau tib neeg kev noj qab haus huv rau tib neeg kev noj qab haus huv.